Extraction, purification, identification and metabolism of 3′,5′-cyclic UMP, 3′,5′-cyclic IMP and 3′,5′-cyclic dTMP from rat tissues
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Extraction, purification, identification and metabolism of 3',5'-cyclic UMP, 3',5'-cyclic IMP and 3',5'-cyclic dTMP from rat tissues.
The large-scale extraction and partial purification of endogenous 3',5'-cyclic UMP, 3',5'-cyclic IMP and 3',5'-cyclic dTMP are described. Rat liver, kidney, heart, spleen and lung tissues were subjected to a sequential purification procedure involving freeze-clamping, perchlorate extraction, alumina and Sephadex ion-exchange chromatography and preparative electrophoresis. The samples thus obtai...
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Chibnall, A. C. & Piper, H. S. (1934). Biochem. J. 28, 2209. Chibnall, A. C., Piper, H. S., Pollard, A., Williams, E. F. & Sahai, P. N. (1934). Biochem. J. 28, 2189. Clark, G. L. & Smith, H. A. (1931). Indu8tr. Engng Chem. 23, 697. Davidson, D. (1940). Indu8tr. Engng Chem. (Anal.) 12, 40. Fetterley, L. C. (1952). U.S. Patent 2,520,715. Herout, V. (1950). Coll. Czech. Chem. Comm. 15, 381. Herout...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1986
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2360431